Human RalA/RalB antibody | NKMAX ATGA0590 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human RalA/RalB antibody

catalog :

ATGA0590

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT41E2

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human RalA/RalB antibody

Catalog number (SKU) :

ATGA0590

NCBI :

NP_005393

Description :

RalA and RalB belong to subfamily of Ras-related GTPases. This protein mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. It is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicletrafficking, and endocytosis. RalA and RalB share 85% identity.

Alternative Names :

Ras-related protein Ral-A, RAL,Ras-related protein Ral-B

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Balasubramanian N., et al. (2009) Curr. Biol. 20:75-79.;;Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.;; Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.;;Jullien-Flores V., et al. (1995). J. Biol. Chem. 270: 22473-22477.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human RALA (1-203aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human RalA protein;;Lane 2.: Recombinant human RalB protein;;The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: HepG2 cell lysate;;Lane 3.: NIH3T3 cell lysate;;Lane 4.: PC3 cell lysate;;Lane 5.: U87MG cell lysate;;{ATGA0590-WB2.jpg}

Reacts With :

Human

Clone Number :

AT41E2

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained with ATGA0590 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Epigenetics and Nuclear Signaling

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P11233

Internal Search Terms :

RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, RalA, Ras-related protein Ral-A, Ras related protein RalA, Ras related protein Ral A, ATGA0590-10ug, ATGA0590-20ug, ATGA0590-50ug, ATGA0590-100ug, ATGA0590-250ug, ATGA0590-500ug, ATGA0590-1mg, ATGA0590-010, ATGA0590-020, ATGA0590-050, ATGA0590-100, ATGA0590-250, ATGA0590-500, ATGA0590-01M

more info or order :

NKMAX product webpage