Human ACAT1 antibody | NKMAX ATGA0583 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ACAT1 antibody

catalog :

ATGA0583

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2C5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ACAT1 antibody

Catalog number (SKU) :

ATGA0583

NCBI :

NP_000010

Description :

ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction. Defects in ACAT1 are a cause of 3-ketothiolase deficiency. 3-ketothiolase deficiency is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded.

Alternative Names :

acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Antonenkov, V.D., et al. (2000) Eur J Biochem 267: 2981-2990.;;Korman, S.H. (2006) Mol Genet Metab 89: 289-299.;;Yamaguchi, S., et al. (1988) J Clin Invest 81(3): 813-817.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ACAT1 (34-427aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained with ATGA0583 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Hep3B cell lysate;;Lane 4.: 293T cell lysate;;Lane 5.: A431 cell lysate;;

Reacts With :

Human

Clone Number :

2C5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Additional Comment Only :

This product was produced from tissue culture supe

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P24752

Internal Search Terms :

ACAT1,ATGA0583-10ug,ATGA0583-20ug,ATGA0583-50ug,ATGA0583-100ug,ATGA0583-250ug,ATGA0583-500ug,ATGA0583-1mg,ATGA0583-10,ATGA0583-20,ATGA0583-50,ATGA0583-100,ATGA0583-250,ATGA0583-500,ATGA0583-1

more info or order :

NKMAX product webpage