Human AGXT antibody | NKMAX ATGA0580 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human AGXT antibody

catalog :

ATGA0580

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2T4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human AGXT antibody

Catalog number (SKU) :

ATGA0580

NCBI :

NP_000021

Description :

The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase.Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used toproduce energy and the basic materials important for cellular activities. AGXT converts a compound calledglyoxylate to glycine, an amino acid that is a building block for making enzymes and other proteins.

Alternative Names :

Serpin B5, Protease inhibitor 5, SERPINB5, alanine-glyoxylate aminotransferase, AGXT1, PH1, AGT,SPT, AGT1, oxalosis I, primary hyperoxaluria type 1, L-alanine: glyoxylate aminotransferase 1,serine:pyruvate aminotransferase, glycolicaciduria

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Donini S, et al., (2009) Biochem J 422(2):265-272.;;Grujic D, et al., (2009) Am J Nephrol 29(2):86-93.;;Williams EL, et al., (2009) Hum Mutat 30(6):910-917.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant AGXT (330-392aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of AGXT in Hep3B cells. The cell was stained with ATGA0580 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AGXT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human AGXT protein;;

Reacts With :

Human

Clone Number :

AT2T4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of AGXT in HeLa cells. The cell was stained with ATGA0580 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Metabolism

Additional Comment Only :

This product was produced from tissue culture supe

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P21549

Internal Search Terms :

AGXT,ATGA0580-10ug,ATGA0580-20ug,ATGA0580-50ug,ATGA0580-100ug,ATGA0580-250ug,ATGA0580-500ug,ATGA0580-1mg,ATGA0580-10,ATGA0580-20,ATGA0580-50,ATGA0580-100,ATGA0580-250,ATGA0580-500,ATGA0580-1

more info or order :

NKMAX product webpage