Human ACAT2 antibody | NKMAX ATGA0579 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ACAT2 antibody

catalog :

ATGA0579

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT55D5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ACAT2 antibody

Catalog number (SKU) :

ATGA0579

NCBI :

NP_005882

Description :

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that inhumans is encoded by the ACAT (acetyl-Coenzyme A acetyltransferase 2) gene. ACAT2 is an acetyl-CoAC-acetyltransferase enzyme, plays a role in regulating lipid metabolism. It catalyzes the synthesis ofacetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids.

Alternative Names :

Acetyl-CoA acetyltransferase cytosolic, ACTL, Acetyl-CoA acetyltransferase, cytosolic

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shintani S., et al. (1999) Genetics. 152(2): 743-754.;;Middleton B., et al. (1974) Biochem J. 139(1): 109-121.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human ACAT2 (1-397aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0579 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: Ramos cell lysate;;The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: ACAT2 Transfected 293T cell lysate;;{ATGA0579-WB2.jpg}

Reacts With :

Human

Clone Number :

AT55D5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cardiovascular

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q9BWD1

Internal Search Terms :

ACAT2,ATGA0579-10ug,ATGA0579-20ug,ATGA0579-50ug,ATGA0579-100ug,ATGA0579-250ug,ATGA0579-500ug,ATGA0579-1mg,ATGA0579-10,ATGA0579-20,ATGA0579-50,ATGA0579-100,ATGA0579-250,ATGA0579-500,ATGA0579-1

more info or order :

NKMAX product webpage