Human PARP2 antibody | NKMAX ATGA0578 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PARP2 antibody

catalog :

ATGA0578

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT29G4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PARP2 antibody

Catalog number (SKU) :

ATGA0578

NCBI :

NP_005475

Description :

PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable ofcatalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to thatof poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminalcatalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of thisprotein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targetingof the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Alternative Names :

Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ame JC, Rolli V, Schreiber V et al. (1999). J. Biol. Chem. 274 (25): 17860-8.;;Schreiber V, Ame JC, Dolle P et al. (2002). J. Biol. Chem. 277 (25): 23028-36.;;Maeda Y, Hunter TC, Loudy DE et al. (2006). J. Biol. Chem. 281 (14): 9600-6.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PARP2 (233-583aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0578 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: SW480 cell lysate;;The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;{ATGA0578-WB2.jpg}The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: NIH3T3 cell lysate;;Lane 1.: Raji cell lysate;;{ATGA0578-WB3.jpg}

Reacts With :

Human

Clone Number :

AT29G4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0578 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Epigenetics and Nuclear Signaling

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q9UGN5

Internal Search Terms :

PARP2,ATGA0578-10ug,ATGA0578-20ug,ATGA0578-50ug,ATGA0578-100ug,ATGA0578-250ug,ATGA0578-500ug,ATGA0578-1mg,ATGA0578-10,ATGA0578-20,ATGA0578-50,ATGA0578-100,ATGA0578-250,ATGA0578-500,ATGA0578-1

more info or order :

NKMAX product webpage