Human Coactosin-like Protein 1/COTL1 antibody | NKMAX ATGA0576 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Coactosin-like Protein 1/COTL1 antibody

catalog :

ATGA0576

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1D6

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Coactosin-like Protein 1/COTL1 antibody

Catalog number (SKU) :

ATGA0576

NCBI :

NP_066972

Description :

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal proteinthat belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified inslime mold and is found at highest levels in kidney, placenta, lung and peripheral blood leukocytes. COTL1 isone of many proteins that participate in regulation of Actin's cytoskeleton through a calcium dependent mechanism,but has not been shown to have a direct effect on Actin depolymerization.

Alternative Names :

Coactosin like F-actin binding protein 1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, CLP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Provost. P., et al. (2001) Biochem J 359(1): 255-263.;;Dai. H., et al. (2006) Biochim Biophys Acta 1764(11): 1688-1700.;;Esser. J., et al. (2010) Biochem J 425(1): 265-274.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human COTL1 (1-142aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;The cell lysates(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2: Coactosin-like Protein 1/COTL1 Transfected 293T cell lysate;;{ATGA0576-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1D6

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Coactosin-like Protein 1/COTL1 in HeLa cells. The cell was stained with ATGA0576 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Signal Transduction

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q14019

Internal Search Terms :

CLP, Coactosin like F-actin binding protein 1, Coactosin like Protein1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, Coactosin-like protein, Coactosin-like Protein 1, Coactosin-like Protein1, COTL 1, COTL1, COTL-1, FLJ43657, MGC19733, ATGA0576-10ug, ATGA0576-20ug, ATGA0576-50ug, ATGA0576-100ug, ATGA0576-250ug, ATGA0576-500ug, ATGA0576-1mg, ATGA0576-010, ATGA0576-020, ATGA0576-050, ATGA0576-100, ATGA0576-250, ATGA0576-500, ATGA0576-01M

more info or order :

NKMAX product webpage