Human Lactate Dehydrogenase A/LDHA antibody | NKMAX ATGA0574 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Lactate Dehydrogenase A/LDHA antibody

catalog :

ATGA0574

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Lactate Dehydrogenase A/LDHA antibody

Catalog number (SKU) :

ATGA0574

NCBI :

NP_005557

Description :

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Alternative Names :

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107(5):2037-42.;; Xie H, et al. (2009) Mol Cancer Ther, 8(3):626-35.;; Toyoda K, et al. (2010) J Bacteriol, 191(13):4251-8.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human LDHA (1-332aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

Flow cytometry analysis of Lactate Dehydrogenase A/LDHA in HeLa cells. The cell was stained with ATGA0574 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: Jurkat cell lysate;;Lane 3.: 293T cell lysate;;Lane 4.: A431 cell lysate;;

Reacts With :

Human

Clone Number :

AT1A4

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00338

Internal Search Terms :

Lactate Dehydrogenase A, LDHA,ATGA0574-10ug,ATGA0574-20ug,ATGA0574-50ug,ATGA0574-100ug,ATGA0574-250ug,ATGA0574-500ug,ATGA0574-1mg,ATGA0574-10,ATGA0574-20,ATGA0574-50,ATGA0574-100,ATGA0574-250,ATGA0574-500,ATGA0574-1

more info or order :

NKMAX product webpage