Human pan-RAS antibody | NKMAX ATGA0573 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human pan-RAS antibody

catalog :

ATGA0573

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2G9

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human pan-RAS antibody

Catalog number (SKU) :

ATGA0573

NCBI :

NP_002515

Description :

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activatedby ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinasestransiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes areHRAS, NRAS and KRAS.

Alternative Names :

Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6,Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N rasprotein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879,Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Rocks O, et al. (2006). Current Opinion in Cell Biology 18 (4): 351-7.;;Wennerberg K, et al. (March 2005). J. Cell. Sci. 118 (Pt 5): 843-6.;;Rotblat B, et al. (2008). Methods Enzymol 439: 467-89.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human NRAS (1-186aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human NRAS protein;;Lane 2.: Recombinant human KRAS protein;;Lane 3.: Recombinant human HRAS protein;;The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;{2004010146591329.jpg}

Reacts With :

Human

Clone Number :

AT2G9

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of RAS in HeLa cells. The cell was stained with ATGA0573 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cancer

Additional Comment Only :

This product was produced from tissue culture supernatant. Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras(cell signaling).

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P01111

Internal Search Terms :

RAS,ATGA0573-10ug,ATGA0573-20ug,ATGA0573-50ug,ATGA0573-100ug,ATGA0573-250ug,ATGA0573-500ug,ATGA0573-1mg,ATGA0573-10,ATGA0573-20,ATGA0573-50,ATGA0573-100,ATGA0573-250,ATGA0573-500,ATGA0573-1

more info or order :

NKMAX product webpage