Human beta-Actin antibody | NKMAX ATGA0570 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human beta-Actin antibody

catalog :

ATGA0570

quantity :

1 ml

price :

174 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4G6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human beta-Actin antibody

Catalog number (SKU) :

ATGA0570

NCBI :

NP_001092

Description :

Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Alternative Names :

Actin, cytoplasmic 1, Beta-actin

Concentration :

0.2mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Gunning PW., et al. (2015) Journal of Cell Science. 128(11): 2009-2019.;;Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163(4): 673-8.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ACTB (1-375aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: Jurkat cell lysate;; Lane 3.: PC3 cell lysate;; Lane 4.: HepG2 cell lysate;; Lane 5.: MCF7 cell lysate;;Lane 6.: U87-MG cell lysate;; Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Kidney tissue lysate;;Lane 2.: Liver tissue lysate;;Lane 3.: Spleen tissue lysate;;Lane 4.: Bone marrow tissue lysate;;Lane 5.: Stomach tissue lysate {ATGA0570-WB2.jpg}The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: ACTB antibody 1:1000;;Lane 2.: ACTB antibody 1:3000;;Lane 3.: ACTB antibody 1:5000;;Lane 4.: ACTB antibody 1:10000 {ATGA0570-WB3.jpg}

Reacts With :

Human

Clone Number :

AT4G6

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P60709

Internal Search Terms :

b-Actin, beta actin, beta-actin, actin, ATGA0457,ATGA0570-10ug,ATGA0570-20ug,ATGA0570-50ug,ATGA0570-100ug,ATGA0570-250ug,ATGA0570-500ug,ATGA0570-1mg,ATGA0570-10,ATGA0570-20,ATGA0570-50,ATGA0570-100,ATGA0570-250,ATGA0570-500,ATGA0570-1

References with this Product :

Yang YJ, et al. Liquid Chromatography/Tandem Mass Spectrometry Analysis of Sophora flavescens Aiton and Protective Effects against Alcohol-Induced Liver Injury and Oxidative Stress in Mice. (Antioxidants (Basel). 2024){https://pubmed.ncbi.nlm.nih.gov/38790646/}

more info or order :

NKMAX product webpage