antibody

Human Hexokinase antibody

ATGA0569

50 ul

190 USD

monoclonal

mouse

nonconjugated

4D7

western blot, ELISA, immunocytochemistry

Human Hexokinase antibody

ATGA0569

NP_000179

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group fromATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for theparticular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous formof hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have beenassociated with hemolytic anemia due to hexokinase deficiency.

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase-A, HK I, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HXK1, mHk1-s, tumor isozyme, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase 4, HK1,HK2, HK3, HK4

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Ellison W R. et al., (1975) J Biol Chem 250:1864-1871.;;Furuta H. et al., (1996) Genomics 36(1):206-9.;;Jon E. et al., (2003) J.Exp Biology 206:2049-2057.

Mouse

IgG2a kappa

Recombinant human Hexokinase1 (1-917aa) purified from E. coli

ELISA, WB, ICC/IF

ICC/IF analysis of Hexokinase in HeLa cells. The cell was stained with ATGA0569 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human Hexokinase1 protein;;Lane 2.: Recombinant human Hexokinase2 protein;;Lane 3.: Recombinant human Hexokinase3 protein;;Lane 4.: Recombinant human Hexokinase4 protein;;The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Jurkat cell lysate;;Lane 4.: K562 cell lysate;;Lane 5.: Mouse brain Tissue lysate{ATGA0569-WB2.jpg}

Human

4D7

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Human

Cancer

This product was produced from tissue culture supernatant.

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal antibody

P19367

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase type III, Hexokinase1, Hexokinase2, Hexokinase3, Hexokinase-1, Hexokinase-2, Hexokinase-3, Hexokinase-A, HK I, HK II, HK III, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HK3, HKI, HKII, HKIII, HXK1, HXK2, HXK3, HXK 1, HXK 2, HXK 3, HXK-1, HXK-2, HXK-3, mHk1-s, Muscle form hexokinase, Tumor isozyme, ATGA0569-10ug, ATGA0569-20ug, ATGA0569-50ug, ATGA0569-100ug, ATGA0569-250ug, ATGA0569-500ug, ATGA0569-1mg, ATGA0569-010, ATGA0569-020, ATGA0569-050, ATGA0569-100, ATGA0569-250, ATGA0569-500, ATGA0569-01M