Human MEMO1 antibody | NKMAX ATGA0566 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MEMO1 antibody

catalog :

ATGA0566

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1E9

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MEMO1 antibody

Catalog number (SKU) :

ATGA0566

NCBI :

NP_057039

Description :

MEMO1 (Mediator of ErbB2-driven cell motility 1) is a 297 amino acid protein. It is thought to relax extracellular chemotactic signals that are targeted at the microtubule cytoskeleton, thereby controlling cell migration. Additionally, MEMO1 is required for breast carcinoma migration, suggesting an important role in tumorigenesis. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity.

Alternative Names :

Protein MEMO1, C2orf4, CGI-27, MEMO, NS5ATP7

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Marone R., et al. (2004) Nat Cell Biol 6(6):515-522.;;Meira M., et al. (2009) J Cell Sci 122:787-797.;;Zaoui K., et al. (2010) Proc Natl Acad Sci USA 107(43):18517-18522.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human MEMO1 (1-297aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: U87MG cell lysate

Reacts With :

Human

Clone Number :

AT1E9

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained with ATGA0566 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Signal Transduction

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q9Y316

Internal Search Terms :

MEMO1,ATGA0566-10ug,ATGA0566-20ug,ATGA0566-50ug,ATGA0566-100ug,ATGA0566-250ug,ATGA0566-500ug,ATGA0566-1mg,ATGA0566-10,ATGA0566-20,ATGA0566-50,ATGA0566-100,ATGA0566-250,ATGA0566-500,ATGA0566-1

more info or order :

NKMAX product webpage