Human NPM1 antibody | NKMAX ATGA0565 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NPM1 antibody

catalog :

ATGA0565

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT23F1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NPM1 antibody

Catalog number (SKU) :

ATGA0565

NCBI :

NP_002511

Description :

NPM1 is associated with nucleolar ribonucleoprotein structures and bind single-stranded and double-strandednucleic acids, but it binds preferentially G-Quadruplex forming nucleic acids. It is involved in the biogenesisof ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation throughSUMOylation (by SENP3 and SENP5) is another facet of the proteins's regulation and cellular functions.It is located in the nucleolus, but it can be translocated to the nucleoplasm in case of serum starvation ortreatment with anticancer drugs. The protein is phosphorylated. Chromosomal aberrations involving NPM1 werefound in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acutemyelogenous leukemia. It has been found in the cytoplasm in patients with primary acute myelogenous leukemia.

Alternative Names :

Nucleophosmin, B23, NPM

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Maiguel D.A., et al.(2004) Mol cell Biol. 24: 3703-3711.;;Colombo E., et al. (2005) Mol cell Biol. 25: 8874-8886.;;

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NPM1(1-294aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of NPM1 in HeLa cells. The cell was stained with ATGA0565 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: HepG2 cell lysate;;Lane 1.: Jurkat cell lysate;;

Reacts With :

Human

Clone Number :

AT23F1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NPM1 in WiDr cells. The cell was stained with ATGA0565 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cancer

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P06748

Internal Search Terms :

NPM1,ATGA0565-10ug,ATGA0565-20ug,ATGA0565-50ug,ATGA0565-100ug,ATGA0565-250ug,ATGA0565-500ug,ATGA0565-1mg,ATGA0565-10,ATGA0565-20,ATGA0565-50,ATGA0565-100,ATGA0565-250,ATGA0565-500,ATGA0565-1

more info or order :

NKMAX product webpage