Human visfatin/NAMPT antibody | NKMAX ATGA0556 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human visfatin/NAMPT antibody

catalog :

ATGA0556

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human visfatin/NAMPT antibody

Catalog number (SKU) :

ATGA0556

NCBI :

NP_005737

Description :

Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

Alternative Names :

Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, PBEF, PBEF1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-31;;Hug C, et al., (2005) Science. 307:366-367

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PBEF/Visfatin (1-491aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PBEF/Visfatin in HeLa cells. The cell was stained with ATGA0556 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PBEF/Visfatin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: SW480 cell lysate;;

Reacts With :

Human

Clone Number :

4D5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cardiovascular

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P43490

Internal Search Terms :

PBEF, Visfatin,ATGA0556-10ug,ATGA0556-20ug,ATGA0556-50ug,ATGA0556-100ug,ATGA0556-250ug,ATGA0556-500ug,ATGA0556-1mg,ATGA0556-10,ATGA0556-20,ATGA0556-50,ATGA0556-100,ATGA0556-250,ATGA0556-500,ATGA0556-1

more info or order :

NKMAX product webpage