EGFP antibody | NKMAX ATGA0553 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

EGFP antibody

catalog :

ATGA0553

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1D9

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

EGFP antibody

Catalog number (SKU) :

ATGA0553

NCBI :

ABW34377

Description :

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

Alternative Names :

Enhanced green fluorescent protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Phillips GJ (Oct 2001) FEMS Microbiology Letters. 204 (1): 9-18.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant EGFP(1-239aa) purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2: EGFP Transfected 293T cell lysate;;

Clone Number :

AT1D9

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Gene Category :

Tags & Cell Markers

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal antibody

Internal Search Terms :

EGFP,ATGA0553-10ug,ATGA0553-20ug,ATGA0553-50ug,ATGA0553-100ug,ATGA0553-250ug,ATGA0553-500ug,ATGA0553-1mg,ATGA0553-10,ATGA0553-20,ATGA0553-50,ATGA0553-100,ATGA0553-250,ATGA0553-500,ATGA0553-1

more info or order :

NKMAX product webpage