antibody

Human Crystallin alpha A/CRYAA antibody

ATGA0550

50 ul

190 USD

monoclonal

mouse

nonconjugated

c9F2

western blot, ELISA

Human Crystallin alpha A/CRYAA antibody

ATGA0550

NP_000385

alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency,which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively.alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP). They actas molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weightaggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunitshave a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and theparticipation in the intracellular architecture.

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Fujii N., et al., (2003) Mol. Vis. 9: 915-22.;;Laksanalamai P., et al., (2004) Extremophiles 8: 1-11.;;

Mouse

IgG1 kappa

Recombinant human AlphaA Crystallin/CRYAA (1-173aa) purified from E. coli

ELISA, WB

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human AlphaA Crystallin/CRYAA protein;;Lane 2.: Recombinant human AlphaB Crystallin/CRYAB protein;;The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: AlphaA Crystallin/CRYAA Transfected 293T cell lysate;;{ATGA0550-WB2.jpg}

Human

c9F2

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Human

Cancer

This product was produced from tissue culture supernatant.

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal antibody

P02489

a A Crystallin, Acry-1, Alpha crystallin A chain, Alpha-crystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, Heat shock protein beta 4, Heat shock protein beta-4, HSPB 4, HspB4, Zonular Central Nuclear Cataract, ATGA0550-10ug, ATGA0550-20ug, ATGA0550-50ug, ATGA0550-100ug, ATGA0550-250ug, ATGA0550-500ug, ATGA0550-1mg, ATGA0550-010, ATGA0550-020, ATGA0550-050, ATGA0550-100, ATGA0550-250, ATGA0550-500, ATGA0550-01M

Oh JY, et al. Identification of the HSPB4/TLR2/NF-κB axis in macrophage as a therapeutic target for sterile inflammation of the cornea. (EMBO Mol Med. 2012){https://pubmed.ncbi.nlm.nih.gov/22359280/};;Fan Q, et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. (Mol Vis. 2014){https://pubmed.ncbi.nlm.nih.gov/24453475/}