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company name :
NKMAX
product type :
antibody
product name :
His-tag antibody
catalog :
ATGA0544
quantity :
50 ul
price :
190 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AT1D6
application :
western blot, ELISA
more info or order :
product information
Product Type :
Antibodies
Item Name :
His-tag antibody
Catalog number (SKU) :
ATGA0544
Description :
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag.
Alternative Names :
6 His epitope tag antibody,Hexa His tag antibody,HHHHHH epitope tag antibody,HHHHHH tag antibody,His tag antibody,Polyhistidine Tag antibody
Concentration :
1mg/ml (determined by BCA assay)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
O Hare, Simura, Gibson; et al. (2006). Biomolecular Methods. 38 (22): 873-885.;;Li Y et al. from the Mariana Trench. Mar Drugs 17:N/A (2019).;;Andersen, Kasper R.; Leksa, Nina C.; Schwartz, Thomas U. (2013).;;
Abs Host :
Mouse
Isotype :
IgG1 kappa
Immunogen :
6x His synthetic peptide
Applications :
ELISA, WB
WB Comment and Pic :
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1: OTUB2-His tag Transfected 293T cell lysate;;Lane 2: GAD-His tag Transfected 293T cell lysate;;The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1: Recombinant human TREM1-His tag protein;;Lane 2: Recombinant human CD34-His tag protein;;Lane 3: Recombinant human ATG3-His tag protein;;{2004010491789039.jpg}
Clone Number :
AT1D6
Ab Usage :
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Gene Category :
Cancer
Conjugation :
Unconjugated
Clonality Mono VS Poly :
Monoclonal
Ab Purification Comment :
By protein-A affinity chromatography
Product Type Name :
Monoclonal antibody
Internal Search Terms :
His tag,ATGA0544-10ug,ATGA0544-20ug,ATGA0544-50ug,ATGA0544-100ug,ATGA0544-250ug,ATGA0544-500ug,ATGA0544-1mg,ATGA0544-10,ATGA0544-20,ATGA0544-50,ATGA0544-100,ATGA0544-250,ATGA0544-500,ATGA0544-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.