Human Caveolin-1 antibody | NKMAX ATGA0540 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Caveolin-1 antibody

catalog :

ATGA0540

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4C1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Caveolin-1 antibody

Catalog number (SKU) :

ATGA0540

NCBI :

NP_001744

Description :

The identification of caveolin-1(CAV1) as the main structural component of caveolae, together with the finding that CAV1 might serve as a molecular organizer for membrane multiprotein complexes involved in cellular traffic, endo-and transcytosis, cell adhesion and signal transduction prompted a new impulse in the research on these intracellular organells. One of the properties of CAV1 was its insolubility in cold non-ionic detergents together with apical markers in epithelial cell. Also, cholesterol is essential for caveolae formation and maintenance, and caveolae structure is highly sensitive to cholesterol depletion or treatment with cholesterol binding drugs. CAV1 tightly and specifically binds free cholesterol and artificial cholesterol containing vesicles.

Alternative Names :

VIP 21, CAV, CAV 1, CAV1, caveolae protein 22 kD, caveolin 1 alpha isoform, caveolin 1 beta isoform, Caveolin 1 caveolae protein 22kDa, Caveolin1, cell growth-inhibiting protein 32, MSTP085, OTTHUMP00000025031, VIP 21, VIP21

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Van Meer, G., et al. (2008) Nat Rev Mol Cell Biol. 9: 112-124;;Todeschini, R.A. et al. (2008) Biochim Biophy Acta 1780: 421-433;;Lajoie, P et al. (2007) J Cell Mol Med 11: 644-653

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CAV1 (1-104aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of CAV1 in A549 cells. The cell was stained with ATGA0540 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse heart tissue lysate

Reacts With :

Human

Clone Number :

AT4C1

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q03135

Internal Search Terms :

Caveolin1,ATGA0540-10ug,ATGA0540-20ug,ATGA0540-50ug,ATGA0540-100ug,ATGA0540-250ug,ATGA0540-500ug,ATGA0540-1mg,ATGA0540-10,ATGA0540-20,ATGA0540-50,ATGA0540-100,ATGA0540-250,ATGA0540-500,ATGA0540-1

more info or order :

NKMAX product webpage