Human MafK antibody | NKMAX ATGA0538 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MafK antibody

catalog :

ATGA0538

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F7

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MafK antibody

Catalog number (SKU) :

ATGA0538

NCBI :

NP_002351

Description :

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locuscontrol regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by thetranscription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of aheterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack aputative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize amongthemselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger)basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerizewith Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteinsis primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

Alternative Names :

Transcription factor MafK, NFE2u, P18

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Oyake, T., et al. (1996) Mol Cell Biol 16(11): 6083-95.;;Toki, T., et al. (1997) Oncogene 14: 1901-1910.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human MAFK (1-156aa) purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MafK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;The recombinant proteins(20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MafK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human MafK protein;;Lane 2.: Recombinant human MafG protein;;Lane 3.: Recombinant human MafF protein;;{2004010481853669.jpg}

Reacts With :

Human

Clone Number :

AT2F7

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenetics and Nuclear Signaling

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

O60675

Internal Search Terms :

MafK,ATGA0538-10ug,ATGA0538-20ug,ATGA0538-50ug,ATGA0538-100ug,ATGA0538-250ug,ATGA0538-500ug,ATGA0538-1mg,ATGA0538-10,ATGA0538-20,ATGA0538-50,ATGA0538-100,ATGA0538-250,ATGA0538-500,ATGA0538-1

more info or order :

NKMAX product webpage