Human MINCLE/CLEC4E antibody | NKMAX ATGA0537 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MINCLE/CLEC4E antibody

catalog :

ATGA0537

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT16E3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MINCLE/CLEC4E antibody

Catalog number (SKU) :

ATGA0537

NCBI :

NP_055173

Description :

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6, 6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

Alternative Names :

CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ishikawa E, et al. (2009) J Exp Med, 206(13):2879-88.;;Matsunaga I, et al. (2009) J Exp Med, 206(13):2865-8.;;Graham LM, et al. (2009) Cytokine, 48(1-2):148-55.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CLEC4E (41-219aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CLEC4E in HeLa cells. The cell was stained with ATGA0537 (1:500). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: CLEC4E Transfected 293T cell lysate;;

Reacts With :

Human

Clone Number :

AT16E3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CLEC4E in LnCap cells. The cell was stained with ATGA0537 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Immunology

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q9ULY5

Internal Search Terms :

CLEC 4E, CLEC4E, CLECSF9, C-type lectin domain family 4, member E, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2, ATGA0537-10ug, ATGA0537-20ug, ATGA0537-50ug, ATGA0537-100ug, ATGA0537-250ug, ATGA0537-500ug, ATGA0537-1mg, ATGA0537-010, ATGA0537-020, ATGA0537-050, ATGA0537-100, ATGA0537-250, ATGA0537-500, ATGA0537-01M

more info or order :

NKMAX product webpage