Human Leptin/OB antibody | NKMAX ATGA0536 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Leptin/OB antibody

catalog :

ATGA0536

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT7E10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Leptin/OB antibody

Catalog number (SKU) :

ATGA0536

NCBI :

NP_000221

Description :

Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarilyin the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated,resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes andinfertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of usingleptin as a therapeutic agent, are currently under intense investigation.

Alternative Names :

LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, LeptinPrecursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin,

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Anini Y., et al. (2003) Diabetes. 52(2): 252-259.;;Jeong KJ., et al. (1999) Appl Environ Microbiol. 65(7): 3027-32.;;Hyogo H., et al. (2002) J. Biol. Chem. 277(37): 34117-34124.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Leptin/OB (22-167aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of Leptin/OB in HeLa cells. The cell was stained with ATGA0536 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Leptin/OB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: Leptin/OB Transfected 293T cell lysate;;

Reacts With :

Human

Clone Number :

AT7E10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Growth factors

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P41159

Internal Search Terms :

Leptin, OB,ATGA0536-10ug,ATGA0536-20ug,ATGA0536-50ug,ATGA0536-100ug,ATGA0536-250ug,ATGA0536-500ug,ATGA0536-1mg,ATGA0536-10,ATGA0536-20,ATGA0536-50,ATGA0536-100,ATGA0536-250,ATGA0536-500,ATGA0536-1

more info or order :

NKMAX product webpage