Human MYL2 antibody | NKMAX ATGA0531 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MYL2 antibody

catalog :

ATGA0531

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3B2

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MYL2 antibody

Catalog number (SKU) :

ATGA0531

NCBI :

NP_000423

Description :

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Alternative Names :

Slow cardiac myosin regulatory light chain 2, MLC2, CMH10, DKFZp779C0562, Slow cardiac myosin regulatory light chain 2, MYL2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2, MLC 2v, MYL 2, Cardiac ventricular myosin light chain 2, RLC of myosin, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2 ventricular cardiac muscle isoform, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Macera MJ, et al., (1992) Genomics. 13(3):829-31.;;Poetter K, et al., (1996) Nat Genet. 13(1):63-9.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human MYL2 (1-166aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Heart Tissue lysate

Reacts With :

Human

Clone Number :

AT3B2

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of MYL2 in A431 cells. The cell was stained with ATGA0531 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Additional Comment Only :

This product was produced from tissue culture supernatant.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P10916

Internal Search Terms :

MYL2,ATGA0531-10ug,ATGA0531-20ug,ATGA0531-50ug,ATGA0531-100ug,ATGA0531-250ug,ATGA0531-500ug,ATGA0531-1mg,ATGA0531-10,ATGA0531-20,ATGA0531-50,ATGA0531-100,ATGA0531-250,ATGA0531-500,ATGA0531-1

References with this Product :

Sapp V, et al. Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity. (Sci Rep. 2021){https://pubmed.ncbi.nlm.nih.gov/34230586/}

more info or order :

NKMAX product webpage