antibody

Human PP2C alpha/PPM1A antibody

ATGA0529

50 ul

190 USD

monoclonal

mouse

nonconjugated

p6c7

western blot, ELISA, immunocytochemistry

Human PP2C alpha/PPM1A antibody

ATGA0529

NP_066283

Protein phosphatase 2C (PP2Calpha) is a Mn2+ - or Mg 2+ -dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNk MAPk pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signalingkinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Calpha, the prototypical member of the PPM family.

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lin X, et al., (2006) Cell. 125(5): 915-928. ;;Duan X, et al., (2006) J Biol Chem. 281(48):36526-32.

Mouse

IgG2b kappa

Recombinant human PP2Calpha/PPM1A (1-382aa) purified from E. coli

ELISA, WB, ICC/IF

ICC/IF analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained with ATGA0529 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha/PPM1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1 : Jurkat cell lysate;;Lane 2 : HeLa cell lysate;;Lane 3 : K-562 cell lysate;;Lane 4 : MCF7 cell lysate;;Lane 5 : A549 cell lysate;;Lane 6 : Raji cell lysate;;Lane 7 : Mouse kidney tissue lysate;; Lane 8 : Mouse brain tissue lysate;;Lane 9 : Mouse liver tissue lysate

Human

p6C7

The antibody has been tested by ELISA , Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Human

Cancer

This product was produced from tissue culture supernatant.

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal antibody

P35813

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2C-alpha, PP2Calpha, PP2CA, PP2C A, PP2C alpha, PDPC 1, PDP1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43, ATGA0529-10ug, ATGA0529-20ug, ATGA0529-50ug, ATGA0529-100ug, ATGA0529-250ug, ATGA0529-500ug, ATGA0529-1mg, ATGA0529-010, ATGA0529-020, ATGA0529-050, ATGA0529-100, ATGA0529-250, ATGA0529-500, ATGA0529-01M

Kim JS, et al. The interaction of hepatitis B virus X protein and protein phosphatase type 2 Calpha and its effect on IL-6. (Biochem Biophys Res Commun. 2006){https://pubmed.ncbi.nlm.nih.gov/17055456/};;Kim YG, et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. (Arthritis Rheumatol. 2014){https://pubmed.ncbi.nlm.nih.gov/24980965/};;Pereira JM, et al. Infection Reveals a Modification of SIRT2 Critical for Chromatin Association. (Cell Rep. 2018){https://pubmed.ncbi.nlm.nih.gov/29694890/}