Human CD158f/KIR2DL5A antibody | NKMAX ATGA0525 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CD158f/KIR2DL5A antibody

catalog :

ATGA0525

quantity :

50 ul

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT11C11

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CD158f/KIR2DL5A antibody

Catalog number (SKU) :

ATGA0525

NCBI :

NP_065396

Description :

KIR/CD158, also known as killer cell immunoglobulin-like receptor 2DL5A, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. It is detected on the cell surface as a monomer that, upon tyrosine phosphorylation, recruits the Srchomology region 2-containing protein tyrosine phosphatase-2, It is thus an inhibitory receptor gathering a combination of genetic, structural, and functional features unique among KIR, which suggests that it plays a specialized role in innate immunity.

Alternative Names :

Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 5A, Killer cell immunoglobulin-like receptor 2DL5A, KIR2DL5A, CD158F, KIR2DL5A.1, KIR2DL5A.3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Vilches, C., et al. (2000) J. Immunol. 164: 5797-5804.;;Elfishawi SM., et al. (2017) Hum. Immunol. 78 (11-12), 684-691.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human KIR/CD158 (22-238aa) purified from E.coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR/CD158 antibody (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: MCF7 cell lysate;;Lane 3.: HepG2 cell lysate;;Lane 4.: K562 cell lysate

Reacts With :

Human

Clone Number :

AT11C11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of KIR/CD158 in PBMC. The cell was stained with ATGA0525 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Other

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q8N109

Internal Search Terms :

KIR, CD158,ATGA0525-10ug,ATGA0525-20ug,ATGA0525-50ug,ATGA0525-100ug,ATGA0525-250ug,ATGA0525-500ug,ATGA0525-1mg,ATGA0525-10,ATGA0525-20,ATGA0525-50,ATGA0525-100,ATGA0525-250,ATGA0525-500,ATGA0525-1

more info or order :

NKMAX product webpage