antibody

Human RAB5A antibody

ATGA0519

50 ul

190 USD

monoclonal

mouse

nonconjugated

3A4

western blot, ELISA, immunocytochemistry

Human RAB5A antibody

ATGA0519

NP_004153

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bucci C, et al., (1992) Cell 70 (5): 715-28;;Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1): 679-85

Mouse

IgG2b kappa

Recombinant human Rab5a (1-215aa) purified from E. coli.

ELISA, WB, ICC/IF

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ATGA0519 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate;; Lane 2.: Rab5a Transfected 293T cell lysate;;

Human

3A4

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Human

Neuroscience

This product was produced from tissue culture supernatant.

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal antibody

P20339

Rab5a,ATGA0519-10ug,ATGA0519-20ug,ATGA0519-50ug,ATGA0519-100ug,ATGA0519-250ug,ATGA0519-500ug,ATGA0519-1mg,ATGA0519-10,ATGA0519-20,ATGA0519-50,ATGA0519-100,ATGA0519-250,ATGA0519-500,ATGA0519-1

Gorji-Bahri G, et al. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. (J Cell Biochem. 2021){https://pubmed.ncbi.nlm.nih.gov/33225526/};;Gorji-Bahri G, et al. RAB5A effect on metastasis of hepatocellular carcinoma cell line via altering the pro-invasive content of exosomes. (Exp Mol Pathol. 2021){https://pubmed.ncbi.nlm.nih.gov/33831402/};;Polla DL, et al. Biallelic variants in TMEM222 cause a new autosomal recessive neurodevelopmental disorder. (Genet Med. 2021){https://pubmed.ncbi.nlm.nih.gov/33824500/};;Nanou A, et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis. (Cell Rep. 2021){https://pubmed.ncbi.nlm.nih.gov/34038728/};;Huang HW, Ryoo HD. Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival. (PLoS Genet. 2021){https://pubmed.ncbi.nlm.nih.gov/34714826/};;Patel M, et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. (Br J Cancer. 2022){https://pubmed.ncbi.nlm.nih.gov/35173303/};;Tang Y, et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. (Acta Pharm Sin B. 2022){https://pubmed.ncbi.nlm.nih.gov/35847504/}