antibody

Human IRF5 antibody

ATGA0518

50 ul

190 USD

monoclonal

mouse

nonconjugated

10T1

western blot, ELISA, immunocytochemistry, FACS

Human IRF5 antibody

ATGA0518

NP_116032.1

Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

interferon regulatory factor 5, irf5

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Mario B. et al. (2007) J. Virology 81(9):4473-4481.;;Ning S. et al. (2005) J. Virology 79:11671-11676.;;Mario B. et al. (2007) J. Virology 81(9):4473-4481.

Mouse

IgG1 kappa

Recombinant human IRF-5 (176-240aa) purified from E. coli

ELISA, WB, ICC/IF, FACS

ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with ATGA0518 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with ATGA0518 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010457189584.jpg}

The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: THP-1 cell lysate

Human

10T1

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of IRF5 in THP-1 cells. The cell was stained with ATGA0518 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Human

Immunology

This product was produced from tissue culture supernatant.

Unconjugated

Monoclonal

Monoclonal Antibody

Q13568

IRF5,ATGA0518-10ug,ATGA0518-20ug,ATGA0518-50ug,ATGA0518-100ug,ATGA0518-250ug,ATGA0518-500ug,ATGA0518-1mg,ATGA0518-10,ATGA0518-20,ATGA0518-50,ATGA0518-100,ATGA0518-250,ATGA0518-500,ATGA0518-1

Moen SH, et al. Human Toll-like Receptor 8 (TLR8) Is an Important Sensor of Pyogenic Bacteria, and Is Attenuated by Cell Surface TLR Signaling. (Front Immunol. 2019){https://pubmed.ncbi.nlm.nih.gov/31214180/};;Guo J, et al. A promising role of interferon regulatory factor 5 as an early warning biomarker for the development of human non-small cell lung cancer. (Lung Cancer. 2019){https://pubmed.ncbi.nlm.nih.gov/31447002/}