Human Actin/ACTA1 antibody | NKMAX ATGA0517 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Actin/ACTA1 antibody

catalog :

ATGA0517

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Actin/ACTA1 antibody

Catalog number (SKU) :

ATGA0517

NCBI :

NP_001091

Description :

ACTA1 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Alternative Names :

ACTA, ACTA1, Actin, alpha 1, Alpha actin 1, Alpha skeletal muscle, Alpha skeletal muscle actin, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3, Skeletal muscle

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Moreno CAM , et al., (2017) Pediatr. Neurol. 75: 11-16. ;;Loumaye A ,et al., (2017) J Cachexia Sarcopenia Muscle 8 (5): 768-777.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human Actin (13-377aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of Actin in HeLa cells. The cell was stained with ATGA0517 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Lung Tissue lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: A549 cell lysate;;Lane 4.: A431 cell lysate;;Lane 5.: Raji cell lysate;;

Reacts With :

Human

Clone Number :

AT2F5

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P68133

Internal Search Terms :

Skeletal muscle, NEM3, NEM2, NEM1, MPFD, CFTDM, CFTD, ASMA, Alpha-actin-1, alpha1, Alpha skeletal muscle actin, Alpha skeletal muscle, Alpha actin 1, alpha 1, Actin, ACTA1, ACTA, ACTA 1, Alpha actin1, NEM 3, NEM 2, NEM 1, Actin, ATGA0517-10ug, ATGA0517-20ug, ATGA0517-50ug, ATGA0517-100ug, ATGA0517-250ug, ATGA0517-500ug, ATGA0517-1mg, ATGA0517-010, ATGA0517-020, ATGA0517-050, ATGA0517-100, ATGA0517-250, ATGA0517-500, ATGA0517-01M

more info or order :

NKMAX product webpage