Human PPAR gamma/NR1C3 antibody | NKMAX ATGA0514 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PPAR gamma/NR1C3 antibody

catalog :

ATGA0514

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4F5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PPAR gamma/NR1C3 antibody

Catalog number (SKU) :

ATGA0514

NCBI :

NP_619725

Description :

PPAR gamma, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. It regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N- (2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.

Alternative Names :

Peroxisome proliferator-activated receptor gamma, NR1C3, CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, PPARG

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Berger J. et al. (2002) Annu Rev Med . 53:409-35. ;;Michalik L .,et al. (2006) Pharmacol Rev. 58(4):726-41.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PPARG (209-477aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of PPAR gamma in HeLa cells. The cell was stained with ATGA0514 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPAR gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate

Reacts With :

Human

Clone Number :

AT4F5

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PPAR gamma in 293T cells. The cell was stained with ATGA0514 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P37231

Internal Search Terms :

PPAR g,ATGA0514-10ug,ATGA0514-20ug,ATGA0514-50ug,ATGA0514-100ug,ATGA0514-250ug,ATGA0514-500ug,ATGA0514-1mg,ATGA0514-10,ATGA0514-20,ATGA0514-50,ATGA0514-100,ATGA0514-250,ATGA0514-500,ATGA0514-1

more info or order :

NKMAX product webpage