Human Iduronate 2-Sulfatase/IDS antibody | NKMAX ATGA0513 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Iduronate 2-Sulfatase/IDS antibody

catalog :

ATGA0513

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6H4

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Iduronate 2-Sulfatase/IDS antibody

Catalog number (SKU) :

ATGA0513

NCBI :

NP_000193

Description :

IDS, as known asiduronate 2-sulfatase isoform, isa member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O-sulfate and N-salfate esters from a varity of substrates. This protein is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate. It hydrolyzes the 2-sulfate group of the IDS units of the GAG.

Alternative Names :

Iduronate 2-sulfatase isoform, IDS, MPS2, SIDS

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Torres LC., et al, (2014) Clin. Immunol. 154:100-104.;; Chistiakov DA., et al, (2014) J.Genet.Genomics. 41:197-203.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human IDS (26-550aa) purified from insect cell

Applications :

ELISA,WB,FACS

WB Comment and Pic :

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human IDS

Reacts With :

Human

Clone Number :

AT6H4

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of IDS in HeLa cells. The cell was stained with ATGA0513 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Antigen Species :

Human

Gene Category :

Other

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P22304

Internal Search Terms :

Iduronate2-Sulfatase, IDS,ATGA0513-10ug,ATGA0513-20ug,ATGA0513-50ug,ATGA0513-100ug,ATGA0513-250ug,ATGA0513-500ug,ATGA0513-1mg,ATGA0513-10,ATGA0513-20,ATGA0513-50,ATGA0513-100,ATGA0513-250,ATGA0513-500,ATGA0513-1

more info or order :

NKMAX product webpage