Human Histone Deacetylase 2/HDAC2 antibody | NKMAX ATGA0510 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Histone Deacetylase 2/HDAC2 antibody

catalog :

ATGA0510

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3B1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Histone Deacetylase 2/HDAC2 antibody

Catalog number (SKU) :

ATGA0510

NCBI :

NP_001518

Description :

HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Alternative Names :

Histone deacetylase 2, HD2, RPD3, YAF1, KDAC2, Protein deacylase HDAC2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Betz R. et al. (1998) Genomics. 52:245-6.;;Taunton J. et al. (1996) Science 272:408-11.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human HDAC2 (1-488aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of HDAC2 in HeLa cells. The cell was stained with ATGA0510 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HDAC2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human HDAC2

Reacts With :

Human

Clone Number :

AT3B1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of HDAC2 in A549 cells. The cell was stained with ATGA0510 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q92769

Internal Search Terms :

Histone Deacetylase2, HDAC2,ATGA0510-10ug,ATGA0510-20ug,ATGA0510-50ug,ATGA0510-100ug,ATGA0510-250ug,ATGA0510-500ug,ATGA0510-1mg,ATGA0510-10,ATGA0510-20,ATGA0510-50,ATGA0510-100,ATGA0510-250,ATGA0510-500,ATGA0510-1

more info or order :

NKMAX product webpage