Human NMT1 antibody | NKMAX ATGA0501 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NMT1 antibody

catalog :

ATGA0501

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2C8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NMT1 antibody

Catalog number (SKU) :

ATGA0501

NCBI :

NP_066565

Description :

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Glover C.J. et al. (1997) J. Biol. Chem. 272:28680-28689.;;Van Damme P. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human NMT1 (1-496aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of NMT1 in HeLa cells. The cell was stained with ATGA0501 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NMT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HepG2 cell lysate ;;Lane 2.: 293T cell lysate

Reacts With :

Human

Clone Number :

AT2C8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30419

Internal Search Terms :

NMT1,ATGA0501-10ug,ATGA0501-20ug,ATGA0501-50ug,ATGA0501-100ug,ATGA0501-250ug,ATGA0501-500ug,ATGA0501-1mg,ATGA0501-10,ATGA0501-20,ATGA0501-50,ATGA0501-100,ATGA0501-250,ATGA0501-500,ATGA0501-1

more info or order :

NKMAX product webpage