HA Tag antibody | NKMAX ATGA0499 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

HA Tag antibody

catalog :

ATGA0499

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5E7

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

HA Tag antibody

Catalog number (SKU) :

ATGA0499

Description :

HA tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a HA-tag allows one to follow the protein with an antibody against the HA epitope.

Alternative Names :

Influenza Hemagglutinin epitope

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Field. J, et al (1988) Mol. Cell. Biol. 8: 2159-2165.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Synthetic peptide(YPYDVPDYA)-KLH

Applications :

ELISA, WB

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate;; Lane 2.: PDPN-HA tag Transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: 293T cell lysate ;;Lane 2.: EPO-HA tag Transfected 293T cell lysate {ATGA0499-WB2.jpg} The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human FABP2-HA tag {ATGA0499-WB3.jpg} The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti HA tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human MIF-HA tag` {ATGA0499-WB4.jpg}

Clone Number :

AT5E7

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Fusion Tag & Detection

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

Internal Search Terms :

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more info or order :

NKMAX product webpage