Human Glutathione S-transferase pi 1/GSTP1 antibody | NKMAX ATGA0498 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Glutathione S-transferase pi 1/GSTP1 antibody

catalog :

ATGA0498

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT12C10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Glutathione S-transferase pi 1/GSTP1 antibody

Catalog number (SKU) :

ATGA0498

NCBI :

NP_000843

Description :

GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.

Alternative Names :

Glutathione S-transferase P, DFN7, FAEES3, GST3, PI, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S Transferase Pi, GSTP1, Glutathione S-Transferase pi 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lee KA, et al. (2001) Blood. 98(12): 3483-5.;; Hayes JD,et al. (1995) Crit Rev BiochemMol Biol. 30(6): 445-600.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GSTP1 (1-210aa) purified from E.coli.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of GSTP1 in A549 cells. The cell was stained with ATGA0498 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: K562 cell lysate ;;Lane 2.: Jurkat cell lysate;; Lane 3.: HeLa cell lysate;; Lane 4.: 293T cell lysate;; Lane 5.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT12C10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GSTP1 in K562 cell line. The cell was stained with ATGA0498 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P09211

Internal Search Terms :

Glutathione S-Transferase pi1, GSTP1,ATGA0498-10ug,ATGA0498-20ug,ATGA0498-50ug,ATGA0498-100ug,ATGA0498-250ug,ATGA0498-500ug,ATGA0498-1mg,ATGA0498-10,ATGA0498-20,ATGA0498-50,ATGA0498-100,ATGA0498-250,ATGA0498-500,ATGA0498-1

more info or order :

NKMAX product webpage