Human CD34 antibody | NKMAX ATGA0497 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CD34 antibody

catalog :

ATGA0497

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3B6

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CD34 antibody

Catalog number (SKU) :

ATGA0497

NCBI :

NP_001764

Description :

CD34, also known as hematopoietic progenitor cell antigen CD34 isoform b, is a member of a family of single-pass transmembrane sialomucin proteins. It is adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. This protein could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.

Alternative Names :

Hematopoietic progenitor cell antigen CD34 isoform b, CD34

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Nielsen JS., et al. (2009) Microcirculation. 16: 487-496.;; Mastrandrea F., et al. (2008) Eur Ann Allergy Clin Immunol. 40: 90-103.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human CD34 (32-290aa) purified from Baculovirus.

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: 293T cell lysate

Reacts With :

Human

Clone Number :

AT3B6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CD34 in KG1 cells. The cell was stained with ATGA0497 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P28906

Internal Search Terms :

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more info or order :

NKMAX product webpage