Human Hexokinase 1/2/3 antibody | NKMAX ATGA0495 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Hexokinase 1/2/3 antibody

catalog :

ATGA0495

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4C12

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Hexokinase 1/2/3 antibody

Catalog number (SKU) :

ATGA0495

NCBI :

NP_002106

Description :

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase 3 may have anabolic functions, providing H6P for glycogen or lipid synthesis.

Alternative Names :

EC 2.7.1.1, Hexokinase 3, Hexokinase type III, HK III, HK3, HXK3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Furuta H. et al.,(1996) Genomics. 36(1): 206-9. ;; Jon E. et al., (2003) J.Exp Biology. 206: 2049-2057.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Hexokinase1,2,3 (1-923aa) purified from E. coli.

Applications :

ELISA, WB

WB Comment and Pic :

The recombianat protein (20ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : Recombinant human Hexokinase 1 ;;Lane 2 : Recombinant human Hexokinase 2 ;;Lane 3 : Recombinant human Hexokinase 3 ;;Lane 4 : Recombinant human Hexokinase 4 The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: Jurkat cell lysate ;;Lane 3.: K562 cell lysate ;;Lane 4.: Mouse brain tissue lysate {ATGA0495-WB2.jpg}

Reacts With :

Human

Clone Number :

AT4C12

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P52790

Internal Search Terms :

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase type III, Hexokinase1, Hexokinase2, Hexokinase3, Hexokinase-1, Hexokinase-2, Hexokinase-3, Hexokinase-A, HK I, HK II, HK III, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HK3, HKI, HKII, HKIII, HXK1, HXK2, HXK3, HXK 1, HXK 2, HXK 3, HXK-1, HXK-2, HXK-3, mHk1-s, Muscle form hexokinase, Tumor isozyme, ATGA0495-10ug, ATGA0495-20ug, ATGA0495-50ug, ATGA0495-100ug, ATGA0495-250ug, ATGA0495-500ug, ATGA0495-1mg, ATGA0495-010, ATGA0495-020, ATGA0495-050, ATGA0495-100, ATGA0495-250, ATGA0495-500, ATGA0495-01M

more info or order :

NKMAX product webpage