Human ATG3 antibody | NKMAX ATGA0493 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ATG3 antibody

catalog :

ATGA0493

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT15F6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ATG3 antibody

Catalog number (SKU) :

ATGA0493

NCBI :

NP_071933

Description :

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy.

Alternative Names :

ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tanida I., et al. (2002) J Biol Chem. 277: 13739-13744. ;; Yamada Y., et al. (2007) J Biol Chem. 282: 8036-8043.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ATG3 (1-314aa) purified from E. coli.

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of ATG3 in HeLa cells. The cell was stained with ATGA0493 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0493-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: ATG3 transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: K562 cell lysate ;;Lane 2.: Raji cell lysate ;;Lane 3.: Jurkat cell lysate {ATGA0493-WB2.jpg}

Reacts With :

Human

Clone Number :

AT15F6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ATG3 in HeLa cells. The cell was stained with ATGA0493 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NT62

Internal Search Terms :

ATG3, ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96,ATGA0493-10ug,ATGA0493-20ug,ATGA0493-50ug,ATGA0493-100ug,ATGA0493-250ug,ATGA0493-500ug,ATGA0493-1mg,ATGA0493-10,ATGA0493-20,ATGA0493-50,ATGA0493-100,ATGA0493-250,ATGA0493-500,ATGA0493-1

more info or order :

NKMAX product webpage