Human VHL antibody | NKMAX ATGA0492 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human VHL antibody

catalog :

ATGA0492

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT82B10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human VHL antibody

Catalog number (SKU) :

ATGA0492

NCBI :

NP_000542

Description :

Von Hippel-Lindau disease (VHL) is a dominant inherited syndrome characterized by the predisposition to develop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas and hemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in the VHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-type allele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet (beta-domain) and a smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL protein is also involved in the degradation of hypoxia-inducible factor (HIF).

Alternative Names :

Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1, HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin binding protein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, von Hippel Lindau syndrome, von Hippel Lindau tumor suppressor, AVH0616

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Latif F., et al. (1993) Science. 260(5112): 1317-20.;; Duan DR., et al. (1995) PNAS. 92(14): 6459-63.;; Maxwell PH., et al. (1999) Nature. 399(6733): 271-5.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human VHL (1-154aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of VHL in Hep3B. The cell was stained with ATGA0492 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Raji cell lysate;;Lane 4.: Jurkat cell lysate;;Lane 5.: A549 cell lysate;;Lane 6.: MCF7 cell lysate;;Lane 7.: PC3 cell lysate;;

Reacts With :

Human

Clone Number :

AT82B10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Additional Comment Only :

AVH0616 has been replaced with a catalog number ATGA0492.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P40337

Internal Search Terms :

VHL,ATGA0492-10ug,ATGA0492-20ug,ATGA0492-50ug,ATGA0492-100ug,ATGA0492-250ug,ATGA0492-500ug,ATGA0492-1mg,ATGA0492-10,ATGA0492-20,ATGA0492-50,ATGA0492-100,ATGA0492-250,ATGA0492-500,ATGA0492-1

more info or order :

NKMAX product webpage