Human MCM7 antibody | NKMAX ATGA0489 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MCM7 antibody

catalog :

ATGA0489

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1G8

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MCM7 antibody

Catalog number (SKU) :

ATGA0489

NCBI :

NP_877577

Description :

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB.

Alternative Names :

DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhou,Y.M., et al. (2012) Liver Int. 32(10), 1505-1509. ;; Liu,Y.Z., et al. (2012) Lung Cancer 77(1), 176-182.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human MCM7 (1-414aa) purified from E. coli.

Applications :

ELISA,WB,FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MCM7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT1G8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of MCM7 in HeLa cells. The cell was stained with ATGA0489 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P33993

Internal Search Terms :

MCM7, DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,ATGA0489-10ug,ATGA0489-20ug,ATGA0489-50ug,ATGA0489-100ug,ATGA0489-250ug,ATGA0489-500ug,ATGA0489-1mg,ATGA0489-10,ATGA0489-20,ATGA0489-50,ATGA0489-100,ATGA0489-250,ATGA0489-500,ATGA0489-1

more info or order :

NKMAX product webpage