Human alpha-Fetoprotein/AFP antibody | NKMAX ATGA0488 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human alpha-Fetoprotein/AFP antibody

catalog :

ATGA0488

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT73E10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human alpha-Fetoprotein/AFP antibody

Catalog number (SKU) :

ATGA0488

NCBI :

NP_001125

Description :

AFP, also known as alpha-fetoprotein, is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. This protein binds to copper, nickel, fatty acids and bilirubin. The main function of this is to prevent the virilization of female fetuses. As it does not bind estrogen, its function in humans is less clear.

Alternative Names :

Alpha-fetoprotein, AFPD, FETA, HPAFP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tomasi TB Jr., (1997) Annu Rev Med. 28: 453-465. ;; Elbrecht A, et al. (1992) Science. 255: 467-470.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human AFP (19-609aa) purified from insect cell.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of AFP in HaLe cells. The cell was stained with ATGA0488 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HepG2 cell lysate ;;Lane 2.: Mouse liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Jurkat cell lysate ;;Lane 2.: Raw264.7 cell lysate {ATGA0488-WB2.jpg}

Reacts With :

Human

Clone Number :

AT73E10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of AFP in HeLa cells. The cell was stained with ATGA0488 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cardiovascular

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P02771

Internal Search Terms :

a-Fetoprotein, AFP,ATGA0488-10ug,ATGA0488-20ug,ATGA0488-50ug,ATGA0488-100ug,ATGA0488-250ug,ATGA0488-500ug,ATGA0488-1mg,ATGA0488-10,ATGA0488-20,ATGA0488-50,ATGA0488-100,ATGA0488-250,ATGA0488-500,ATGA0488-1

more info or order :

NKMAX product webpage