Human FHL2 antibody | NKMAX ATGA0486 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human FHL2 antibody

catalog :

ATGA0486

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT21D11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human FHL2 antibody

Catalog number (SKU) :

ATGA0486

NCBI :

NP_001034581

Description :

FHL2 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Alternative Names :

Four and a half LIM domains 2, AAG11, DRAL, FHL-2, SLIM-3, SLIM3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Verset,L., et al. (2013) Br. J. Cancer. 109(1), 114-120. ;; Han,W., et al. (2013) J. Clin. Invest. 123(5), 2103-2118.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human FHL2 (1-279aa) purified from E. coli.

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of FHL2 in HeLa cells. The cell was stained with ATGA0486 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0486-IF1b.jpg}

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FHL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: A549 cell lysate ;;Lane 3.: MCF7 cell lysate ;;Lane 4.: SK-OV-3 cell lysate ;;Lane 5.: HT1080 cell lysate

Reacts With :

Human

Clone Number :

AT21D11

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q14192

Internal Search Terms :

FHL2, Four and a half LIM domains 2, AAG11, DRAL, FHL-2, SLIM-3, SLIM3,ATGA0486-10ug,ATGA0486-20ug,ATGA0486-50ug,ATGA0486-100ug,ATGA0486-250ug,ATGA0486-500ug,ATGA0486-1mg,ATGA0486-10,ATGA0486-20,ATGA0486-50,ATGA0486-100,ATGA0486-250,ATGA0486-500,ATGA0486-1

more info or order :

NKMAX product webpage