Human PEDF/SERPINF1 antibody | NKMAX ATGA0484 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PEDF/SERPINF1 antibody

catalog :

ATGA0484

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT13D9

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PEDF/SERPINF1 antibody

Catalog number (SKU) :

ATGA0484

NCBI :

NP_002606.3

Description :

Pigment epithelium-derived factor (PEDF) is a 50kDa glycoprotein and member of the serpin superfamily without any activity of serine protease. PEDF is an angiogenesis inhibitor with neurotrophic properties, balances angiogenesis in the eye and blocks tumor progression. That is, in neuronal cell, PEDF is promoting cell survival and differentiation, however, when endothelial cells are exposed to PEDF, their cell migration and proliferation are inhibited, and they undergo apoptosis.

Alternative Names :

SERPINF1, Serpin peptidase inhibitor clade F member 1, Proliferation inducing protein 35, Pigment epithelium-derived factor EPC 1, Pigment epithelium-derived factor, PIG35, PI, GSTP1, GST3, Glutathione S-Transferase Pi 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S-transferase P, Glutathione S Transferase Pi, FAEES3, EPC1, DFN7, APF0619

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Baba H. et al (2005) Arteriosler Thromb Vasc Biol. 25: 1938-1944.;; Filleur S. et al (2005) Cancer Res. 65: 5144-5152.;; Hattenbach LO. et al (2004) Ophthalmic Res. 37: 341-346.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PEDF (20-418aa) purified from E. coli.

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: PEDF transfected 293T cell lysate

Reacts With :

Human

Clone Number :

AT13D9

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PEDF in K562 cells. The cell was stained with ATGA0484 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Additional Comment Only :

APF0619 has been replaced with a catalog number ATGA0484.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P36955

Internal Search Terms :

EPC1, EPC-1, Glutathione S-Transferase pi1, GSTP1, IL 1 A, IL 1 alpha, IL 1A, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1alpha, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, PEDF, PIG 35, PIG35, Pigment epithelium derived factor, Pigment epithelium derived factor EPC 1, Pigment epithelium-derived factor, Pigment epithelium-derived factor EPC 1, Proliferation inducing protein 35, Serpin F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, SERPINF1, SERPIN-F1, ATGA0484-10ug, ATGA0484-20ug, ATGA0484-50ug, ATGA0484-100ug, ATGA0484-250ug, ATGA0484-500ug, ATGA0484-1mg, ATGA0484-010, ATGA0484-020, ATGA0484-050, ATGA0484-100, ATGA0484-250, ATGA0484-500, ATGA0484-01M

more info or order :

NKMAX product webpage