Human Calnexin antibody | NKMAX ATGA0483 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Calnexin antibody

catalog :

ATGA0483

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT18B9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Calnexin antibody

Catalog number (SKU) :

ATGA0483

NCBI :

NP_001019820

Description :

Calnexin, also known as IP90, p88 and p90, is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, endoplasmic reticulum (ER) -associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Alternative Names :

CNX, IP90, P90, CANX, FLJ26570, Histocompatibility complex class I antigen binding protein p88

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wang B., et al. (2009) Immunology. 128(1): 43-57.;; Millar DJ., et al. (2009) Proteomics. 9(9): 2355-72.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Calnexin (21-481aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of Calnexin in HeLa cells. The cell was stained with ATGA0483 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calnexin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: MCF7 cell lysate ;;Lane 3.: A431 cell lysate ;;Lane 4.: A549 cell lysate;;Lane 5.: 293T cell lysate;;Lane 6.: HepG2 cell lysate;;Lane 7.: Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT18B9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Calnexin in HeLa cells. The cell was stained with ATGA0483 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Flow cytometry analysis of Calnexin in A549 cells. The cell was stained with ATGA0483 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).{ATGA0483-FC2.jpg}

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P27824

Internal Search Terms :

Calnexin,ATGA0483-10ug,ATGA0483-20ug,ATGA0483-50ug,ATGA0483-100ug,ATGA0483-250ug,ATGA0483-500ug,ATGA0483-1mg,ATGA0483-10,ATGA0483-20,ATGA0483-50,ATGA0483-100,ATGA0483-250,ATGA0483-500,ATGA0483-1

more info or order :

NKMAX product webpage