Human MCM7 antibody | NKMAX ATGA0481 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MCM7 antibody

catalog :

ATGA0481

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT118H7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MCM7 antibody

Catalog number (SKU) :

ATGA0481

NCBI :

NP_877577

Description :

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB.

Alternative Names :

DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhou Y.M., et al. (2012) Liver Int. 32(10): 1505-1509.;; Liu,Y.Liu Y.Z., et al. (2012) Lung Cancer. 77(1): 176-182.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human MCM7 (1-414aa) purified from E. coli.

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of MCM7 in MCF7 cells. The cell was stained with ATGA0481 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0481-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MCM7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: Jurkat cell lysate ;;Lane 3.: K562 cell lysate

Reacts With :

Human

Clone Number :

AT118H7

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P33993

Internal Search Terms :

MCM7, DNA replication licensing factor MCM7 isoform 2, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,ATGA0481-10ug,ATGA0481-20ug,ATGA0481-50ug,ATGA0481-100ug,ATGA0481-250ug,ATGA0481-500ug,ATGA0481-1mg,ATGA0481-10,ATGA0481-20,ATGA0481-50,ATGA0481-100,ATGA0481-250,ATGA0481-500,ATGA0481-1

more info or order :

NKMAX product webpage