Human Akt1 antibody | NKMAX ATGA0480 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Akt1 antibody

catalog :

ATGA0480

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6D5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Akt1 antibody

Catalog number (SKU) :

ATGA0480

NCBI :

NP_001014432

Description :

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Alternative Names :

Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lindhurst MJ, et al (2011) N Engl J Med. 365(7): 611-9.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human AKT1 (1-480aa) purified from insect cell.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0480-IF1b.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: NIH3T3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human AKT1 ;;Lane 2.: Recombinant Human AKT2 ;;Lane 3.: Recombinant Human AKT3 {ATGA0480-WB2.jpg}

Reacts With :

Human

Clone Number :

AT6D5

Ab Usage :

The antibody has been tested by Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P31749

Internal Search Terms :

Akt1, RAC-alpha serine/threonine-protein kinase, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA,ATGA0480-10ug,ATGA0480-20ug,ATGA0480-50ug,ATGA0480-100ug,ATGA0480-250ug,ATGA0480-500ug,ATGA0480-1mg,ATGA0480-10,ATGA0480-20,ATGA0480-50,ATGA0480-100,ATGA0480-250,ATGA0480-500,ATGA0480-1

more info or order :

NKMAX product webpage