Human Angiopoietin-like Protein 3/ANGPTL3 antibody | NKMAX ATGA0478 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Angiopoietin-like Protein 3/ANGPTL3 antibody

catalog :

ATGA0478

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT23D8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Angiopoietin-like Protein 3/ANGPTL3 antibody

Catalog number (SKU) :

ATGA0478

NCBI :

NP_055310

Description :

ANGPTL3 also known as Angiopoietin-related protein 3 is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN) -like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis.

Alternative Names :

ANG-5, Angiopoietin 5, Angiopoietin-related protein 3, ANGPT5, ANGPTL3, ANL3, FHBL2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Arca M., et al. (2013) Curr. Opin. Lipidol. 24(4): 313-20.;; Quagliarini F Q., et al. (2012) Proc Natl Acad Sci. 109(48): 19751-6.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human ANGPT5 (243-460aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of ANGPT5 in HeLa cells. The cell was stained with ATGA0478 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ANGPT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Mouse kidney tissue lysate

Reacts With :

Human

Clone Number :

AT23D8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Additional Comment Only :

AAP0501 has been replaced with a catalog number ATGA0478.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9Y5C1

Internal Search Terms :

Angiopoietin-like Protein3, ANGPTL3, Angiopoietin-related protein 3, ANG-5, ANGPT5, ANL3, FHBL2,ATGA0478-10ug,ATGA0478-20ug,ATGA0478-50ug,ATGA0478-100ug,ATGA0478-250ug,ATGA0478-500ug,ATGA0478-1mg,ATGA0478-10,ATGA0478-20,ATGA0478-50,ATGA0478-100,ATGA0478-250,ATGA0478-500,ATGA0478-1

more info or order :

NKMAX product webpage