Human DKK3 antibody | NKMAX ATGA0476 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human DKK3 antibody

catalog :

ATGA0476

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT103B5

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human DKK3 antibody

Catalog number (SKU) :

ATGA0476

NCBI :

NP_001018067

Description :

Dickkopf-related protein 3, also known as DKK3, is a member of the DKK protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. DKK3 is a 350 amino acid secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12 amino acid linker region. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Alternative Names :

Dickkopf WNT signaling pathway inhibitor 3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, REIC, RIG, CRRL

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cadigan K M., et al. (1997) Genes Dev. 11: 3286-3305.;; Gilnka A., et al. (1998) Nature. 391: 357-362.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human DKK3 (22-350aa) purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DKK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: HepG2 cell lysate;;Lane 3.: MCF7 cell lysate;;Lane 4.: A549 cell lysate;;Lane 5.: Mouse brain tissue lysate;;Lane 6.: Mouse kidney tissue lysate

Reacts With :

Human

Clone Number :

AT103B5

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9UBP4

Internal Search Terms :

Cardiomyocyte regeneration-related lncRNA, CRRL, Dickkopf, Dickkopf 3 homolog, Dickkopf WNT signaling pathway inhibitor 3, Dickkopf-3, Dickkopf-related protein 3, Dickkopf-related protein 3 precursor, DKK3, Dkk-3, Regulated in glioma, REIC, RIG, Xenopus laevis, Xenopus laevis homolog 3, ATGA0476-10ug, ATGA0476-20ug, ATGA0476-50ug, ATGA0476-100ug, ATGA0476-250ug, ATGA0476-500ug, ATGA0476-1mg, ATGA0476-010, ATGA0476-020, ATGA0476-050, ATGA0476-100, ATGA0476-250, ATGA0476-500, ATGA0476-01M

more info or order :

NKMAX product webpage