Human NM23-H2/NME2 antibody | NKMAX ATGA0475 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NM23-H2/NME2 antibody

catalog :

ATGA0475

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2E3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NM23-H2/NME2 antibody

Catalog number (SKU) :

ATGA0475

NCBI :

NP_001018149

Description :

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways.

Alternative Names :

NME/NM23 nucleoside diphosphate kinase 2, Nucleoside diphosphate kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, NDK B, NDP kinase B, NM23B, NDPKB

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Munkonge FM., et al. (2009) J Biol Chem. 284(39): 26978-87.;; Treharne KJ., et al. (2009) FEBS Lett. 583(17): 2789-92.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NME2 (1-152aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of NME2 in HeLa cells. The cell was stained with ATGA0475 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: A549 cell lysate;;Lane 3.: MCF7 cell lysate;;Lane 4.: LnCap cell lysate The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: NME2 transfected 293T cell lysate {ATGA0475-WB2.jpg}

Reacts With :

Human

Clone Number :

AT2E3

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P22392

Internal Search Terms :

NM23-H2,ATGA0475-10ug,ATGA0475-20ug,ATGA0475-50ug,ATGA0475-100ug,ATGA0475-250ug,ATGA0475-500ug,ATGA0475-1mg,ATGA0475-10,ATGA0475-20,ATGA0475-50,ATGA0475-100,ATGA0475-250,ATGA0475-500,ATGA0475-1

more info or order :

NKMAX product webpage