Human Glutamine Synthetase antibody | NKMAX ATGA0474 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Glutamine Synthetase antibody

catalog :

ATGA0474

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8D7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Glutamine Synthetase antibody

Catalog number (SKU) :

ATGA0474

NCBI :

NP_001028216

Description :

Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency.

Alternative Names :

Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Vermeulen T., et al, (2008) Arch Biochem Biophys. 478: 96-102. ;; Liaw SH., et al. (1995) Protein Sci. 4(11): 2358-65.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GLUL (1-373aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of GLUL in HeLa cells. The cell was stained with ATGA0474 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0474-IF1b.jpg}

WB Comment and Pic :

The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GLUL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Mouse brain tissue lysate ;;Lane 2.: Mouse liver tissue lysate

Reacts With :

Human

Clone Number :

AT8D7

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P15104

Internal Search Terms :

GLUL, Glutamine synthetase , GLNS, GS, PIG43, PIG59, cell proliferation-inducing protein 59, GLNA, GLNA_HUMAN, GLNS, GLULGlutamate ammonia ligase, Glutamate decarboxylaseGlutamate--ammonia ligase, glutamine synthaseGlutamine synthetase, GS, PIG 43, PIG 59, PIG43PIG59, Proliferation inducing protein 43,ATGA0474-10ug,ATGA0474-20ug,ATGA0474-50ug,ATGA0474-100ug,ATGA0474-250ug,ATGA0474-500ug,ATGA0474-1mg,ATGA0474-10,ATGA0474-20,ATGA0474-50,ATGA0474-100,ATGA0474-250,ATGA0474-500,ATGA0474-1

more info or order :

NKMAX product webpage