Human Cyclophilin 40/PPID antibody | NKMAX ATGA0473 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cyclophilin 40/PPID antibody

catalog :

ATGA0473

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1B8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cyclophilin 40/PPID antibody

Catalog number (SKU) :

ATGA0473

NCBI :

NP_005029

Description :

Cyclophilin D (also known as peptidylpropyl isomerase D, PPID) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression suppresses the apoptosis in cancer cells.

Alternative Names :

Peptidyl-prolyl cis-trans isomerase D, Peptidylprolyl isomerase D, 40 kDa peptidyl-prolyl cis-trans isomerase, PPIase D, Cyclophilin-related protein, Rotamase D, CYP-40, CypD, Cyclophilin D

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

MachidaK.,et al.(2006) J. Biol. Chem. 281(20): 14314-20.;; Yokoi H.,et al.(1996) Genomics. 35(3): 448-55.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PPID (1-370aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PPID in Hep3B cells. The cell was stained with ATGA0473 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: K562 cell lysate;;Lane 2.: Jurkat cell lysate;;Lane 3.: HeLa cell lysate;;Lane 4.: HepG2 cell lysate;;Lane 5.: A549 cell lysate;;Lane 6.: MCF7 cell lysate;;Lane 7.: SK-OV-3 cell lysate;;Lane 8.: PC3 cell lysate

Reacts With :

Human

Clone Number :

AT1B8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q08752

Internal Search Terms :

PPID,ATGA0473-10ug,ATGA0473-20ug,ATGA0473-50ug,ATGA0473-100ug,ATGA0473-250ug,ATGA0473-500ug,ATGA0473-1mg,ATGA0473-10,ATGA0473-20,ATGA0473-50,ATGA0473-100,ATGA0473-250,ATGA0473-500,ATGA0473-1

more info or order :

NKMAX product webpage