Human Chitinase 3-like 2/CHI3L2 antibody | NKMAX ATGA0472 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Chitinase 3-like 2/CHI3L2 antibody

catalog :

ATGA0472

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2B5

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Chitinase 3-like 2/CHI3L2 antibody

Catalog number (SKU) :

ATGA0472

NCBI :

NP_003991

Description :

CHI3L2, also known as chitinase-3-like protein 2, belongs to chitinase protein family. It is secreted by synovial fibroblasts, chondrocytes, and TGFb/IL4 stimulated monocyte derived macrophages. It has been proposed as a potential biomarker for osteoarthritis.

Alternative Names :

CHI3L2, CHIL2, YKL-39, YKL39

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hu B., et al. (1996) J. Biol. Chem. 271: 19415-19420.;; Gratchev A., et al. (2008) Biomarker Insights. 3: 39-44.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CHI3L2 (27-390aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: HepG2 cell lysate;;Lane 4.: LnCap cell lysate

Reacts With :

Human

Clone Number :

AT2B5

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CHI3L2 in HeLa cells. The cell was stained with ATGA0472 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Stem cell

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q15782

Internal Search Terms :

Chitinase3-like2,ATGA0472-10ug,ATGA0472-20ug,ATGA0472-50ug,ATGA0472-100ug,ATGA0472-250ug,ATGA0472-500ug,ATGA0472-1mg,ATGA0472-10,ATGA0472-20,ATGA0472-50,ATGA0472-100,ATGA0472-250,ATGA0472-500,ATGA0472-1

more info or order :

NKMAX product webpage