Human RAB7A antibody | NKMAX ATGA0471 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human RAB7A antibody

catalog :

ATGA0471

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT10E4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human RAB7A antibody

Catalog number (SKU) :

ATGA0471

NCBI :

NP_004628

Description :

Ras-related protein Rab-7a, also known as RAB7A, is a member of the Ras superfamily of small Rab GTPases. RAB7A associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes. RAB7A has also been shown to regulate growth receptor endocytic trafficking, degradation, maturation of phagosome and autophagic vacuoles.

Alternative Names :

Ras-related protein Rab-7a, RAB7, RAB7A member RAS oncogene family, CMT2B, Charcot-Marie-Tooth neuropathy 2B

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Feng Y., et al. (1995) J Cell Biol. 131: 1435-52.;; Barbero P., et al. (2002) J Cell Biol. 156: 511-8.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human RAB7A (1-207aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAB7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: Jurkat cell lysate;;Lane 3.: MCF7 cell lysate;;Lane 4.: PC3 cell lysate;;Lane 5.: 293T cell lysate;;Lane 6.: SK-OV-3 cell lysate

Reacts With :

Human

Clone Number :

AT10E4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Tags & Cell Markers

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P51149

Internal Search Terms :

Rab7a,ATGA0471-10ug,ATGA0471-20ug,ATGA0471-50ug,ATGA0471-100ug,ATGA0471-250ug,ATGA0471-500ug,ATGA0471-1mg,ATGA0471-10,ATGA0471-20,ATGA0471-50,ATGA0471-100,ATGA0471-250,ATGA0471-500,ATGA0471-1

more info or order :

NKMAX product webpage